individual pcr reactions per chip Search Results


p19 arf  (Novus Biologicals)
93
Novus Biologicals p19 arf
a – c , Bone tissue sections from 9-month-old AD and WT mice were stained for SA-βGal and with Oil Red O. Representative images are shown in a ; quantification of Oil Red O⁺ BMAds per mm² ( b ) and percent SA-βGal⁺ BMAds ( c ). Scale bar, 50 μm; n = 6. d – g , Bone tissue sections from AD and WT mice were subjected to RNAscope using probes for Cdkn2a (p16) and Cdkn1a (p21). Representative images are shown in d , f , with quantification of p16⁺ ( e ) and p21⁺ ( g ) signal intensity. Scale bar, 50 μm; n = 6. h , RT–qPCR analysis of bone marrow cells from AD and WT mice showing relative mRNA expression levels of p16Ink4a , p19Arf and p21Cip ( CDKN1A ). i – k , Bone tissue sections from AD and WT mice were analyzed by RNAscope using probes for Cdkn2a <t>(p19)</t> and H2ax . Representative images are shown in i , with quantification of p19⁺ ( j ) and H2AX⁺ ( k ) mean signal intensity. Scale bar, 50 μm; n = 3. l – n , Bone tissue sections from 24-month-old (old) and 4-month-old (young) mice were subjected to RNAscope. Representative images are shown in l ; quantification of p19⁺ ( m ) and H2AX⁺ ( n ) signal intensity. Scale bar, 50 μm; n = 3. o – r , Double-immunofluorescence staining of tibial sections from AD and WT mice was performed using antibodies specific to perilipin and p19 <t>ARF</t> or γH2AX. Representative images of perilipin⁺p19⁺ cells ( o ) and perilipin⁺γH2AX⁺ cells ( q ), with quantification shown in p , r , respectively. s , t , Similar staining of bone sections from old and young mice using perilipin- and γH2AX-specific antibodies; representative images and quantification shown in s , t . Scale bar, 50 μm; n = 6. Data are shown as mean ± s.e.m. P < 0.05, P < 0.01, P < 0.001, P < 0.0001; statistical analysis via unpaired two-tailed Student’s t -test (two groups) or two-way ANOVA (four groups). Exact P values are available in the .
P19 Arf, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/individual+pcr+reactions+per+chip/p14ARF%2FCDKN2A+Antibody+-+BSA+Free/pmc12443615-338-24-26
Average 93 stars, based on 1 article reviews
p19 arf - by Bioz Stars, 2026-10
93/100 stars
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individual pcr reactions per chip  (fluidigm)
93
fluidigm individual pcr reactions per chip
a – c , Bone tissue sections from 9-month-old AD and WT mice were stained for SA-βGal and with Oil Red O. Representative images are shown in a ; quantification of Oil Red O⁺ BMAds per mm² ( b ) and percent SA-βGal⁺ BMAds ( c ). Scale bar, 50 μm; n = 6. d – g , Bone tissue sections from AD and WT mice were subjected to RNAscope using probes for Cdkn2a (p16) and Cdkn1a (p21). Representative images are shown in d , f , with quantification of p16⁺ ( e ) and p21⁺ ( g ) signal intensity. Scale bar, 50 μm; n = 6. h , RT–qPCR analysis of bone marrow cells from AD and WT mice showing relative mRNA expression levels of p16Ink4a , p19Arf and p21Cip ( CDKN1A ). i – k , Bone tissue sections from AD and WT mice were analyzed by RNAscope using probes for Cdkn2a <t>(p19)</t> and H2ax . Representative images are shown in i , with quantification of p19⁺ ( j ) and H2AX⁺ ( k ) mean signal intensity. Scale bar, 50 μm; n = 3. l – n , Bone tissue sections from 24-month-old (old) and 4-month-old (young) mice were subjected to RNAscope. Representative images are shown in l ; quantification of p19⁺ ( m ) and H2AX⁺ ( n ) signal intensity. Scale bar, 50 μm; n = 3. o – r , Double-immunofluorescence staining of tibial sections from AD and WT mice was performed using antibodies specific to perilipin and p19 <t>ARF</t> or γH2AX. Representative images of perilipin⁺p19⁺ cells ( o ) and perilipin⁺γH2AX⁺ cells ( q ), with quantification shown in p , r , respectively. s , t , Similar staining of bone sections from old and young mice using perilipin- and γH2AX-specific antibodies; representative images and quantification shown in s , t . Scale bar, 50 μm; n = 6. Data are shown as mean ± s.e.m. P < 0.05, P < 0.01, P < 0.001, P < 0.0001; statistical analysis via unpaired two-tailed Student’s t -test (two groups) or two-way ANOVA (four groups). Exact P values are available in the .
Individual Pcr Reactions Per Chip, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/individual+pcr+reactions+per+chip/48%2E770+Digital+Array+IFC+for+Digital+PCR/pm22702367-201-7-13
Average 93 stars, based on 1 article reviews
individual pcr reactions per chip - by Bioz Stars, 2026-10
93/100 stars
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chi-square test  (CH Instruments)
90
CH Instruments chi-square test
a – c , Bone tissue sections from 9-month-old AD and WT mice were stained for SA-βGal and with Oil Red O. Representative images are shown in a ; quantification of Oil Red O⁺ BMAds per mm² ( b ) and percent SA-βGal⁺ BMAds ( c ). Scale bar, 50 μm; n = 6. d – g , Bone tissue sections from AD and WT mice were subjected to RNAscope using probes for Cdkn2a (p16) and Cdkn1a (p21). Representative images are shown in d , f , with quantification of p16⁺ ( e ) and p21⁺ ( g ) signal intensity. Scale bar, 50 μm; n = 6. h , RT–qPCR analysis of bone marrow cells from AD and WT mice showing relative mRNA expression levels of p16Ink4a , p19Arf and p21Cip ( CDKN1A ). i – k , Bone tissue sections from AD and WT mice were analyzed by RNAscope using probes for Cdkn2a <t>(p19)</t> and H2ax . Representative images are shown in i , with quantification of p19⁺ ( j ) and H2AX⁺ ( k ) mean signal intensity. Scale bar, 50 μm; n = 3. l – n , Bone tissue sections from 24-month-old (old) and 4-month-old (young) mice were subjected to RNAscope. Representative images are shown in l ; quantification of p19⁺ ( m ) and H2AX⁺ ( n ) signal intensity. Scale bar, 50 μm; n = 3. o – r , Double-immunofluorescence staining of tibial sections from AD and WT mice was performed using antibodies specific to perilipin and p19 <t>ARF</t> or γH2AX. Representative images of perilipin⁺p19⁺ cells ( o ) and perilipin⁺γH2AX⁺ cells ( q ), with quantification shown in p , r , respectively. s , t , Similar staining of bone sections from old and young mice using perilipin- and γH2AX-specific antibodies; representative images and quantification shown in s , t . Scale bar, 50 μm; n = 6. Data are shown as mean ± s.e.m. P < 0.05, P < 0.01, P < 0.001, P < 0.0001; statistical analysis via unpaired two-tailed Student’s t -test (two groups) or two-way ANOVA (four groups). Exact P values are available in the .
Chi Square Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/individual+pcr+reactions+per+chip/chi+square+test/pm20929385-70-15-19
Average 90 stars, based on 1 article reviews
chi-square test - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


a – c , Bone tissue sections from 9-month-old AD and WT mice were stained for SA-βGal and with Oil Red O. Representative images are shown in a ; quantification of Oil Red O⁺ BMAds per mm² ( b ) and percent SA-βGal⁺ BMAds ( c ). Scale bar, 50 μm; n = 6. d – g , Bone tissue sections from AD and WT mice were subjected to RNAscope using probes for Cdkn2a (p16) and Cdkn1a (p21). Representative images are shown in d , f , with quantification of p16⁺ ( e ) and p21⁺ ( g ) signal intensity. Scale bar, 50 μm; n = 6. h , RT–qPCR analysis of bone marrow cells from AD and WT mice showing relative mRNA expression levels of p16Ink4a , p19Arf and p21Cip ( CDKN1A ). i – k , Bone tissue sections from AD and WT mice were analyzed by RNAscope using probes for Cdkn2a (p19) and H2ax . Representative images are shown in i , with quantification of p19⁺ ( j ) and H2AX⁺ ( k ) mean signal intensity. Scale bar, 50 μm; n = 3. l – n , Bone tissue sections from 24-month-old (old) and 4-month-old (young) mice were subjected to RNAscope. Representative images are shown in l ; quantification of p19⁺ ( m ) and H2AX⁺ ( n ) signal intensity. Scale bar, 50 μm; n = 3. o – r , Double-immunofluorescence staining of tibial sections from AD and WT mice was performed using antibodies specific to perilipin and p19 ARF or γH2AX. Representative images of perilipin⁺p19⁺ cells ( o ) and perilipin⁺γH2AX⁺ cells ( q ), with quantification shown in p , r , respectively. s , t , Similar staining of bone sections from old and young mice using perilipin- and γH2AX-specific antibodies; representative images and quantification shown in s , t . Scale bar, 50 μm; n = 6. Data are shown as mean ± s.e.m. P < 0.05, P < 0.01, P < 0.001, P < 0.0001; statistical analysis via unpaired two-tailed Student’s t -test (two groups) or two-way ANOVA (four groups). Exact P values are available in the .

Journal: Nature Aging

Article Title: Serum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis

doi: 10.1038/s43587-025-00924-z

Figure Lengend Snippet: a – c , Bone tissue sections from 9-month-old AD and WT mice were stained for SA-βGal and with Oil Red O. Representative images are shown in a ; quantification of Oil Red O⁺ BMAds per mm² ( b ) and percent SA-βGal⁺ BMAds ( c ). Scale bar, 50 μm; n = 6. d – g , Bone tissue sections from AD and WT mice were subjected to RNAscope using probes for Cdkn2a (p16) and Cdkn1a (p21). Representative images are shown in d , f , with quantification of p16⁺ ( e ) and p21⁺ ( g ) signal intensity. Scale bar, 50 μm; n = 6. h , RT–qPCR analysis of bone marrow cells from AD and WT mice showing relative mRNA expression levels of p16Ink4a , p19Arf and p21Cip ( CDKN1A ). i – k , Bone tissue sections from AD and WT mice were analyzed by RNAscope using probes for Cdkn2a (p19) and H2ax . Representative images are shown in i , with quantification of p19⁺ ( j ) and H2AX⁺ ( k ) mean signal intensity. Scale bar, 50 μm; n = 3. l – n , Bone tissue sections from 24-month-old (old) and 4-month-old (young) mice were subjected to RNAscope. Representative images are shown in l ; quantification of p19⁺ ( m ) and H2AX⁺ ( n ) signal intensity. Scale bar, 50 μm; n = 3. o – r , Double-immunofluorescence staining of tibial sections from AD and WT mice was performed using antibodies specific to perilipin and p19 ARF or γH2AX. Representative images of perilipin⁺p19⁺ cells ( o ) and perilipin⁺γH2AX⁺ cells ( q ), with quantification shown in p , r , respectively. s , t , Similar staining of bone sections from old and young mice using perilipin- and γH2AX-specific antibodies; representative images and quantification shown in s , t . Scale bar, 50 μm; n = 6. Data are shown as mean ± s.e.m. P < 0.05, P < 0.01, P < 0.001, P < 0.0001; statistical analysis via unpaired two-tailed Student’s t -test (two groups) or two-way ANOVA (four groups). Exact P values are available in the .

Article Snippet: Bone sections were blocked in PBS with 3% BSA for 1 h and then stained overnight (>8 h) with individual primary antibodies specific to p19 ARF (Novus, NB200-111, 1:100), γH2AX (Cell Signaling, 20E3, 1:200), perilipin (Cell Signaling, 9349, 1:200) and perilipin (Sigma, P1873, 1:500) and antibodies recognizing human APP (6E10, Novus, NBP2-62566, 1:200) and human Aβ (clone H31L21, Thermo Fisher Scientific 700254, 1:100).

Techniques: Staining, RNAscope, Quantitative RT-PCR, Expressing, Double Immunofluorescence Staining, Two Tailed Test

a , Schematic showing four CEBPα binding sites (F1–F4) in the p19 ARF -encoding gene. ChIP–qPCR assays using CEBPα or control immunoglobulin G (IgG) antibodies were performed on BMAds from AD and WT mice ( b , c ) and from 24-month-old (old) and 4-month-old (young) mice ( d , e ). RNA Pol II was used as a positive control. Representative agarose gels are shown in b , d ; qPCR fold enrichment at binding sites is shown in c , e . f , Schematic showing four predicted CEBPα-binding sites in the p16 INK4a -encoding gene. ORF, open reading frame; TSS, transcription start site. g , ChIP–qPCR assays using anti-CEBPα or control IgG antibodies were performed on BMAds from AD and WT mice. Two independent experiments yielded consistent results; one is shown. Data are presented as mean ± s.e.m. P < 0.05, P < 0.01, P < 0.001, P < 0.0001 (unpaired two-tailed Student’s t -test or two-way ANOVA). Exact P values are available in the .

Journal: Nature Aging

Article Title: Serum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis

doi: 10.1038/s43587-025-00924-z

Figure Lengend Snippet: a , Schematic showing four CEBPα binding sites (F1–F4) in the p19 ARF -encoding gene. ChIP–qPCR assays using CEBPα or control immunoglobulin G (IgG) antibodies were performed on BMAds from AD and WT mice ( b , c ) and from 24-month-old (old) and 4-month-old (young) mice ( d , e ). RNA Pol II was used as a positive control. Representative agarose gels are shown in b , d ; qPCR fold enrichment at binding sites is shown in c , e . f , Schematic showing four predicted CEBPα-binding sites in the p16 INK4a -encoding gene. ORF, open reading frame; TSS, transcription start site. g , ChIP–qPCR assays using anti-CEBPα or control IgG antibodies were performed on BMAds from AD and WT mice. Two independent experiments yielded consistent results; one is shown. Data are presented as mean ± s.e.m. P < 0.05, P < 0.01, P < 0.001, P < 0.0001 (unpaired two-tailed Student’s t -test or two-way ANOVA). Exact P values are available in the .

Article Snippet: Bone sections were blocked in PBS with 3% BSA for 1 h and then stained overnight (>8 h) with individual primary antibodies specific to p19 ARF (Novus, NB200-111, 1:100), γH2AX (Cell Signaling, 20E3, 1:200), perilipin (Cell Signaling, 9349, 1:200) and perilipin (Sigma, P1873, 1:500) and antibodies recognizing human APP (6E10, Novus, NBP2-62566, 1:200) and human Aβ (clone H31L21, Thermo Fisher Scientific 700254, 1:100).

Techniques: Binding Assay, ChIP-qPCR, Control, Positive Control, Two Tailed Test